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Product Description
TREX1 Antibody | 4969 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen: TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1.
The immunogen is located within amino acids 220 - 270 of TREX1.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 33, 35, 41 kDa
Observed: 31 kDa
Validation: N/A
Isoform: N/A
Purification: TREX1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TREX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TREX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TREX1 Antibody: CRV, AGS1, DRN3, HERNS, Three prime repair exonuclease 1, DNase III
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TREX1 Antibody: Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR) , a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.
