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Product Description
Vinculin Antibody | 7809 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen: Anti-Vinculin antibody (7809) was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. <
Research Area: Homeostasis
Tested Application: E, WB, ICC, IF
Application: WB: 0.5-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3: Cat. No. 1282 - 3T3/NIH Cell Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 124kD
Observed: 124kD
Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies.
Isoform: Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD) , isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD) . Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD) . 7809 can detect human, mouse and rat.
Purification: Vinculin antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Vinculin Antibody: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1) . Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2) . Expression of vinculin were shown to be affected by the level of actin expression (2, 3) . Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4) . Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5) .
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