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Product Description
WAPL Antibody | 7799 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL.
The immunogen is located within amino acids 30 - 80 of WAPL.
Research Area: Homeostasis, Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificiy: WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Positive Control 1: Cat. No. 1288 - A20 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 131 kDa
Observed: 140 kDa
Validation: N/A
Isoform: N/A
Purification: WAPL antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: WAPL antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: WAPL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: WAPL Antibody: FOE, WAPL, KIAA0261, FOE, Wings apart-like protein homolog, Friend of EBNA2 protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: WAPL (wings apart-like) , also known as WAPAL or FOE, is a 1, 190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1) . It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1, 2, 4) . WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3, 4) . WAPL is a new regulator of the development and metastasis of cancerous tissue (4, 5) .
