Euro
USD
British Pound
NULL
Product Description
WSB1 Antibody | 8161 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (82%)
Immunogen: WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1.
The immunogen is located within amino acids 70 - 120 of WSB1.
Research Area: Homeostasis, Cancer
Tested Application: E, WB, IHC-P, IF
Application: WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: WSB1 antibody is human specific.
Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 46 kDa
Observed: 46 kDa
Validation: N/A
Isoform: N/A
Purification: WSB1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: WSB1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: WSB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: WD repeat and SOCS box containing 1, SOCS box-containing WD protein SWiP-1, SWIP1, WD repeat and SOCS box-containing protein 1, WSB-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1) , is a member of the WD-protein subfamily (1) . It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1, 2) . WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3) . Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4, 5) .
