Product Description
AFAP1L Antibody | 6559 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (79%)
Immunogen: AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.
The immunogen is located within amino acids 70 - 120 of AFAP1L.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, ICC, IF
Application: AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: Cat. No. 17-003 - A549 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: AFAP1L Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: AFAP1L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: AFAP1L Antibody: Actin filament-associated protein 1-like 1, AFAP1-like protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1) -like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4) . Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.