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Product Description
ATG4C Antibody | 7907 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C.
The immunogen is located within the last 50 amino acids of ATG4C.
Research Area: Autophagy
Tested Application: E, WB, IHC-P, IF
Application: ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 50 kDa
Observed: 57 kDa
Validation: N/A
Isoform: N/A
Purification: ATG4C antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ATG4C antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ATG4C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: ATG4C Antibody: APG4C, AUTL1, AUTL3, APG4-C, APG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1, 2) . ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3) . Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4) .
