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Product Description
DOK1 Antibody | 1107 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.
The immunogen is located within amino acids 410 - 460 of DOK1.
Research Area: Signal Transduction, Cancer
Tested Application: E, WB, ICC, IF
Application: DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 3: Cat. No. 1204 - K562 Cell Lysate
Positive Control 4: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 62 kDa
Validation: N/A
Isoform: N/A
Purification: DOK1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DOK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: DOK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: DOK1 Antibody: TP1, TLP1, p240, TROVE1, VAULT2, Docking protein 1, Downstream of tyrosine kinase 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
