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Product Description
ERAP1 Antibody | 7467 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (78%)
Immunogen: ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP.
The immunogen is located within amino acids 730 - 780 of ERAP1 .
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificiy: ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.
Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 104 kDa
Observed: 105kDa
Validation: N/A
Isoform: N/A
Purification: ERAP1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ERAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: ERAP1 Antibody : ALAP, A-LAP, ARTS1, ERAAP, APPILS, ARTS-1, ERAAP1, PILSAP, PILS-AP, KIAA0525, UNQ584/PRO1154, Endoplasmic reticulum aminopeptidase 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) , a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1, 2) . It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP) , puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3) . ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3, 4) .
