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Product Description
IKK beta Antibody | 2121 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rat: (95%) , Mouse: (95%)
Immunogen: IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920) , which differs from corresponding murine sequence by one amino acid.
The immunogen is located within amino acids 650 - 700 of IKK beta.
Research Area: Signal Transduction
Tested Application: E, WB, ICC, IF
Application: IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μg/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: This polyclonal antibody has no cross response to IKKa or IKKy.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3: Cat. No. 17-001 - HeLa Cell Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 87 kDa
Validation: N/A
Isoform: N/A
Purification: IKK beta Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: IKK beta Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: IKK beta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: IKK beta Antibody: IKK2, IKKB, IMD15, NFKBIKB, IKK-beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, I-kappa-B-kinase beta
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: IKK beta Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK) , which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ) . IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-alpha and IκB-beta. IKKβ is expressed in variety of human tissues.
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