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Product Description
IL-32 Antibody | 3749 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Anti-IL-32 antibody (3749) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32.
The immunogen is located within the last 50 amino acids of IL-32.
Research Area: Signal Transduction, Cancer
Tested Application: E, WB, IHC-P, IF
Application: WB: 0.5-4 μg/mL; IHC: 2-10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: This antibody detects all isoforms of IL-32.
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 26kD
Observed: 26 kD
Validation:
Recombinant Protein Test (Figure 1) : Anti-IL-32 antibodies (3749) detected human IL-32 recombinant protein at different concentrations.
Isoform: Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27kD) , isoform 2 (188aa, 22kD) , isoform 3 (178aa, 21kD) , isoform 4 (131aa, 15kD) , isoform 5 (168aa, 19kD) , isoform 6 (179aa, 21kD) and isoform 7 (148aa, 17kD) . 3749 can detect all human isoforms.
Purification: IL-32 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: IL-32 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
