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Product Description
MYD88 Antibody | 2125 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Sheep: (100%) , Bovine: (100%) , Chicken: (100%)
Immunogen: Anti-MYD88 antibody (2125) was raised against a peptide corresponding to 16 amino acids near the center of human MYD88 isoform 1.
The immunogen is located within amino acids 220 - 270 of MYD88.
Research Area: Signal Transduction, Innate Immunity
Tested Application: E, WB, IHC-P, IF, IP
Application: WB: 1 - 2 μg/mL; IHC-P: 2 μg/mL; IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry, Immunoprecipitation and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 1203 - A549 Cell Lysate
Positive Control 3: Cat. No. 1204 - K562 Cell Lysate
Positive Control 4: Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 5: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 6: N/A
Molecular Weight: Predicted: 35kD
Observed: 35kD
Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar MYD88 expression profile in both human and mouse cell lines detected by two independent anti-MYD88 antibodies that recognize different epitopes, 2125 against internal domain and 2127 against the C-terminus domain. MYD88 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Additionally, Figure 2 shows the mouse MYD88 protein in NIH/3T3 cells migrates slightly faster than human isoform 1 detected by both MYD88 antibodies (2125 and 2127) , which is well correlated with their calculated molecular masses (33.8 kDa vs 35.4 kDa) .
Independent Antibody Validation in Human Tissues (Figure 3) shows similar MYD88 expression profile in human tissues detected by two independent anti-MYD88 antibodies (2125 and 2127) . MYD88 proteins are detected by the two independent antibodies in liver, kidney, lung, thymus, colon, bladder and breast of human tissues at different expression levels, but not in heart, brain, skin and pancreas.
Animal Species Reactivity (Figure 4) : Anti-MYD88 antibodies (2125 and 2127) can detect the expression of MYD88 protein in the liver and spleen of all tissues and mouse heart, but not in human heart. Additionally, Figure 4 also shows MYD88 protein detected by both MYD88 antibodies (2125 and 2127) in human liver and Daudi cells migrates slightly slower than that in the tissues of mouse and rat, which is well correlated with their calculated molecular masses (35.4 kDa vs 33.8 kDa and 33.9kD) .
siRNA knockdown validation (Figure 5) : Anti-MYD88 antibody (2125) specificity was further verified by MYD88 specific siRNA knockdown. MYD88 signal in HeLa cells transfected with MYD88 siRNAs was weaker in comparison with that in HeLa cells transfected with control siRNAs.
Isoform: Human MYD88 has 7 isoforms, including isoform 1 (317aa, 35.4kD) , isoform 2 (296aa, 33.2 kD) , isoform 3 (251aa, 28.3kD) , isoform 4 (191aa, 20.8kD) , isoform 5 (146aa, 15.8kD) , isoform 6 (275aa, 31.5kD) , and isoform 7 (304aa, 34.1kD) . This antibody detects human isoform 1, 2, 3, 6, 7, but not isoform 4, 5. Mouse MYD88 has two isoforms, including isoform 1 (296aa, 33.8kD) and isoform 2 (250aa, 28.7kD) . Rat MYD88 has only one isoform identified so far (296aa, 33.9kD) .
Purification: MYD88 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: MYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: MYD88 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: MYD88 Antibody: Myeloid differentiation primary response 88, MYD88D
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: MYD88, myeloid differentiation primary response 88, was identified as an innate immune signal transduction adaptor involved in the Toll-like receptor (TLR) and interleukin-1 (IL-1) signaling pathway (1, 2, 3) and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 functions as an adaptor protein for TLRs and IL-1 receptors, which stimulates IRAKs, IRF7 and TRAF6, leading to NF-κB activation, cytokine secretion and inflammatory response (2, 4, 5, 6) . Nuclear factor-kappa-B activation modulates multiple genes regulating the body's immune reactions and inflammatory responses. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation (4, 5) . MyD88 is also employed as a regulator molecule by IL-18 receptor. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs TLR2 and TLR4 as the signaling receptors (7, 8) . MyD88 gene is expressed in many tissues.
