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Product Description
NOX4 Antibody | 7927 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.
The immunogen is located within amino acids 70 - 120 of NOX4.
Research Area: Immunology
Tested Application: E, WB, IHC-P, IF
Application: NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 64 kDa
Observed: 68 kDa
Validation: N/A
Isoform: N/A
Purification: NOX4 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: NOX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1) . NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2, 3) . Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4) . The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
