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Product Description
PIK3R4 Antibody | 7949 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.
The immunogen is located within amino acids 1290 - 1340 of PIK3R4.
Research Area: Autophagy
Tested Application: E, WB, IHC-P, IF
Application: PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: PIK3R4 antibody is human, mouse and rat reactive.
Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 149 kDa
Observed: 140 kDa
Validation: N/A
Isoform: N/A
Purification: PIK3R4 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PIK3R4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1, 2) . PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1) . PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5) .
