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Product Description
PLA1A Antibody | 7059 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (75%) , Bovine: (71%)
Immunogen: PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A.
The immunogen is located within amino acids 380 - 430 of PLA1A.
Research Area: Immunology
Tested Application: E, WB, IF
Application: PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 50 kDa
Validation: N/A
Isoform: N/A
Purification: PLA1A Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PLA1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: PLA1A Antibody: PSPLA1, PS-PLA1, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.
