Product Description
PPM1D Antibody | 8043 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D.
The immunogen is located within the last 50 amino acids of PPM1D.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, IHC-P, IF
Application: PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.
Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 67 kDa
Observed: 68 kDa
Validation: N/A
Isoform: N/A
Purification: PPM1D antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PPM1D antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: PPM1D antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Protein phosphatase Mg2+/Mn2+ dependent 1D, p53-induced protein phosphatase 1, PP2C-DELTA, Protein phosphatase 1D, WIP1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D) , a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1, 2) . Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2, 3) . PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4, 5) .