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Product Description
SGK1 Antibody | 6319 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (89%) , Chicken: (84%) , Rabbit: (89%)
Immunogen: SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1.
The immunogen is located within amino acids 370 - 420 of SGK1.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P, IF
Application: SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist
Positive Control 1: Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2: Cat. No. 10-801 - Human Stomach Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: SGK1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SGK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SGK1 antibody can be stored at 4 ˚C for 3 months and -20 ˚C stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SGK1 Antibody: SGK, SGK
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SGK1 Antibody: Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
