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Product Description
SHANK3 Antibody | 6835 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.
The immunogen is located within amino acids 890 - 940 of SHANK3.
Research Area: Neuroscience
Tested Application: E, WB, IHC-P, IF
Application: SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: SHANK3 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SHANK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SHANK3 Antibody: PSAP2, SCZD15, PROSAP2, SPANK-2, DEL22q13.3, KIAA1650, PSAP2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2, Shank3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3) , a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.
