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Product Description
TRIM21 Antibody | 7729 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Bovine (73%) , Mouse (69%) and Rat (69%) .
Immunogen: Anti-TRIM21 antibody (7729) was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21.
The immunogen is located within amino acids 290-340 of TRIM21.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: WB: 0.5-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4: Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 54kD
Observed: 54 kD
Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein. TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Isoform: Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD) . Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD) . 7729 can detect human and possibly mouse and rat.
Purification: TRIM21 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TRIM21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1) . RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2) . TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3) .
