Product Description
TSLP Antibody | 4023 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Anti-TSLP antibody (4023) was raised against a peptide corresponding to 19 amino acids near the center of human TSLP.
The immunogen is located within the amino acids 40-90 of TSLP.
Research Area: Immunology
Tested Application: E, WB, IHC-P, IF
Application: WB: 0.25 - 4 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human and mouse samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1288 - A20 Cell Lysate
Positive Control 2: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 18 kD
Observed: 17, 24 kD (Post-modifications: 2 N linked glycosylations)
Validation:
Recombinant Protein Test (Figure 2) : Anit-TSLP antibodies (4023) detected human TSLP recombinant protein at different concentrations.
Regulated Expression Validation (Figure 8-11) : Anit-TSLP antibodies (4021) detected increased expression in multiple tissue or cells after treatment with cytokines or chemicals, in which the increase in the expression was inhibited by co-treatment with anti-cholinergic drugs (Figure 8) .
Isoform: Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD) . Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD) . 4023 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.
Purification: TSLP Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TSLP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TSLP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TSLP Antibody: Thymic stromal lymphopoietin
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.