Product Description
Unc93b Antibody | 4553 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Unc93b antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Unc93b.
The immunogen is located within amino acids 80 - 130 of Unc93b.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: Unc93b antibody can be used for the detection of Unc93b by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Multiple isoforms of Unc93b are known to exist. This antibody will not cross-react with Unc93a.
Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2: Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 56, 66 kDa
Validation: N/A
Isoform: N/A
Purification: Unc93b Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Unc93b Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Unc93b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Unc93b Antibody: IIAE1, UNC93, UNC93B, Unc-93B1, Protein unc-93 homolog B1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Unc93b Antibody: The endoplasmic reticulum (ER) protein Unc93b, a human homolog of the C. elegans Unc93 gene, was initially identified by a forward genetic screen using N-ethyl-N-nitrosourea where a histidine-to-arginine substitution in Unc93b caused defects in Toll-like receptor (TLR) 3, 7 and 9 signaling. Unlike Unc93a, another homolog of the C. elegans Unc93 gene whose function is unknown, Unc93b specifically interacts with TLR3, 7 and 9; the histidine-to-arginine point mutation used to identify Unc93b abolishes this interaction. Mice carrying this point mutation are highly susceptible to infection with a number of viruses, indicating that Unc93b plays an important role in innate immunity.