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Product Description
RHOG Antibody | 7981 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (87%) , Guinea pig: (87%) , Chicken: (87%) , Pig: (71%)
Immunogen: RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG.
The immunogen is located within amino acids 40 - 90 of RHOG.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P
Application: RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificiy: RHOG antibody is human, mouse and rat reactive.
Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 21 kDa
Observed: 23 kDa
Validation: N/A
Isoform: N/A
Purification: RHOG antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RHOG antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RHOG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Ras homolog family member G, Rho-related GTP-binding protein, ARHG
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The ras homolog family member G (RHOG) , also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1) . RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2) . RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3) .
