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Product Description
Wnt10b Antibody | 4619 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b.
The immunogen is located within amino acids 130 - 180 of Wnt10b.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P
Application: Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
Positive Control 1: Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 43 kDa
Observed: 47 kDa
Validation: N/A
Isoform: N/A
Purification: Wnt10b Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Wnt10b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Wnt10b Antibody: SHFM6, WNT-12, WNT12, Protein Wnt-10b, Protein Wnt-12
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
