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Product Description
HARS Antibody | 62-997 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen: This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS.
Research Area: Immunology
Tested Application: WB, IHC-P
Application: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 57 kDa
Validation: N/A
Isoform: N/A
Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality: Polyclonal
Clone: N/A
Isotype: Rabbit Ig
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration: batch dependent
Storage Condition: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
