Euro
USD
British Pound
NULL
![Recombinant CD45RB Antibody [PTPRC/1783R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575616/812599/porsci_lo__79508.1648973713__00090.1649091983.png?c=2 "Recombinant CD45RB Antibody [PTPRC/1783R]")
![Recombinant CD45RB Antibody [PTPRC/1783R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575616/812599/porsci_lo__79508.1648973713__00090.1649091983.png?c=2 "Recombinant CD45RB Antibody [PTPRC/1783R]")
Product Description
Recombinant CD45RB Antibody [PTPRC/1783R] | 33-819 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Human B lymphocytes were used as immunogen for this recombinant CD45RB antibody.
Research Area: Cancer, Immunology, Neuroscience, Signal Transduction
Tested Application: Flow, IHC-P, IF, WB
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistology (FFPE) : 0.5-1 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Western blot: 0.5-1 ug/ml
Due to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein A affinity chromatography
Clonality: Recombinant Monoclonal
Clone: PTPRC/1783R
Isotype: IgG, k
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4) , CD45RB (exon 5) , CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.
![Recombinant CD45RB Antibody [PTPRC/1783R]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575616/812599/porsci_lo__79508.1648973713__00090.1649091983.png?c=2 "Recombinant CD45RB Antibody [PTPRC/1783R]")
![CD45RB Antibody [PTPRC/1132]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575154/811505/porsci_lo__79508.1648973713__80904.1649091841.png?c=2 "CD45RB Antibody [PTPRC/1132]")
![CD45RB Antibody [PTPRC/1147]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575157/811512/porsci_lo__79508.1648973713__78596.1649091842.png?c=2 "CD45RB Antibody [PTPRC/1147]")
