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Product Description
TRIM5 alpha Antibody | 3251 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha.
The immunogen is located within amino acids 390 - 440 of TRIM5 alpha.
Research Area: Infectious Disease
Tested Application: E, WB, IHC-P
Application: TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.
Positive Control 1: Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2: Cat. No. 1317 - Human Uterus Tissue Lysate
Positive Control 3: Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 4: Cat. No. 11-401 - Human Uterus Tissue Slide
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 54 kDa
Observed: 54 kDa
Validation: N/A
Isoform: N/A
Purification: TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TRIM5 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
