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Product Description
UNG1 Antibody | 3863 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat (85%) .
Immunogen: Anti-UNG1 antibody (3863) was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human UNG1.
The immunogen is located within the last 50 amino acids of UNG1.
Research Area: Neuroscience, Infectious Disease
Tested Application: E, WB, IHC-P, IF
Application: WB: 0.5-4 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1285 - C2C12 Cell Lysate
Positive Control 2: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3: Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 34kD
Observed: 34 kD
Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar UNG1 expression profile in human cell lines detected by two independent anti-UNG1 antibodies that recognize different epitopes, 3863 against C-terminus domain and 3865 against central domain. UNG1 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies.
Isoform: Human UNG1 has 2 isoforms, including isoform 1 (304aa, 34kD) and isoform 2 (313aa, 35kD) . Mouse UNG1 has 2 isoforms, including isoform 1 (295aa, 33kD) and isoform 2 (306aa, 34kD) . Rat UNG1 has one isoform (303aa, 34kD) . 3863 can detect human, mouse and rat.
Purification: UNG1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: UNG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: UNG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: UNG1 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5, UNG15, DGU, Uracil-DNA glycosylase
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.
