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![Apaf-1 Antibody [2E10]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/552398/759499/porsci_lo__79508.1648973713__61035.1649084804.png?c=2 "Apaf-1 Antibody [2E10]")
![Apaf-1 Antibody [2E10]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/552398/759499/porsci_lo__79508.1648973713__61035.1649084804.png?c=2 "Apaf-1 Antibody [2E10]")
Product Description
Apaf-1 Antibody [2E10] | PM-2015 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Research Area: Apoptosis
Tested Application: E, WB, IHC-P
Application: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1204 - K562 Cell Lysate
Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 130 kDa
Validation: N/A
Isoform: N/A
Purification: Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Clonality: Monoclonal
Clone: 2E10
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Apaf-1 monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: Apaf-1 Antibody [2E10] : CED4, APAF-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3) , which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
![Apaf-1 Antibody [2E10]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552398/759499/porsci_lo__79508.1648973713__61035.1649084804.png?c=2 "Apaf-1 Antibody [2E10]")
![Apaf-1 Antibody [5E11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552399/759502/porsci_lo__79508.1648973713__50162.1649084804.png?c=2 "Apaf-1 Antibody [5E11]")
