Euro
USD
British Pound
NULL
![Bcl-2 Antibody [124]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/574942/811002/porsci_lo__79508.1648973713__35041.1649091765.png?c=2 "Bcl-2 Antibody [124]")
![Bcl-2 Antibody [124]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/574942/811002/porsci_lo__79508.1648973713__35041.1649091765.png?c=2 "Bcl-2 Antibody [124]")
Product Description
Bcl-2 Antibody [124] | 33-070 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Research Area: Apoptosis, Autophagy, Cancer
Tested Application: WB, Flow, IHC, E, IF
Application: ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: Does not react with mouse and rat
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: 124
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
![Bcl-2 Antibody [124]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/574942/811002/porsci_lo__79508.1648973713__35041.1649091765.png?c=2 "Bcl-2 Antibody [124]")
![Bcl-2 Antibody [100/D5]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/574941/810999/porsci_lo__79508.1648973713__13883.1649091765.png?c=2 "Bcl-2 Antibody [100/D5]")