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![Beta-2 Microglobulin Antibody [B2M/961]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575298/811835/porsci_lo__79508.1648973713__08778.1649091886.png?c=2 "Beta-2 Microglobulin Antibody [B2M/961]")
![Beta-2 Microglobulin Antibody [B2M/961]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575298/811835/porsci_lo__79508.1648973713__08778.1649091886.png?c=2 "Beta-2 Microglobulin Antibody [B2M/961]")
Product Description
Beta-2 Microglobulin Antibody [B2M/961] | 33-458 | ProSci
Host: Mouse
Reactivity: Human, Non-Human primate
Homology: N/A
Immunogen: Full length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody.
Research Area: Immunology
Tested Application: Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: B2M/961
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: B2M Antibody: Beta-2-microglobulin
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
![Beta-2 Microglobulin Antibody [B2M/961]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575298/811835/porsci_lo__79508.1648973713__08778.1649091886.png?c=2 "Beta-2 Microglobulin Antibody [B2M/961]")
