Product Description
CD2 Antibody [HuLy-m1] | 33-483 | ProSci
Host: Mouse
Reactivity: Human, Cat
Homology: N/A
Immunogen: Human thymocytes were used as the immunogen for the CD2 antibody.
Research Area: Other
Tested Application: Func, Flow, IF, WB
Application: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 1-2 ug/ml
Optimal dilution of the CD2 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: HuLy-m1
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: CD2, CD2 antigen, Erythrocyte receptor, LFA-3 receptor, LFA-2, Lymphocyte-function antigen-2, T11, SRBC, CD2 molecule, Rosette receptor, T-cell surface antigen CD2
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.