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![CD45RA Antibody [SPM504]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575156/811510/porsci_lo__79508.1648973713__40420.1649091842.png?c=2 "CD45RA Antibody [SPM504]")
![CD45RA Antibody [SPM504]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575156/811510/porsci_lo__79508.1648973713__40420.1649091842.png?c=2 "CD45RA Antibody [SPM504]")
Product Description
CD45RA Antibody [SPM504] | 33-299 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.
Research Area: Cancer, Immunology, Neuroscience, Signal Transduction
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
Immunofluorescence: 1-2 ug/ml
Immunohistology (FFPE) : 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SPM504
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve) .
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