Euro
USD
British Pound
NULL
![CD74 Antibody [LN-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/574972/811067/porsci_lo__79508.1648973713__82089.1649091773.png?c=2 "CD74 Antibody [LN-2]")
![CD74 Antibody [LN-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/574972/811067/porsci_lo__79508.1648973713__82089.1649091773.png?c=2 "CD74 Antibody [LN-2]")
Product Description
CD74 Antibody [LN-2] | 33-103 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody.
Research Area: Other
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: LN-2
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) , HLA-DR-gamma, Ia-associated invar
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage) , macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .
![CD74 Antibody [LN-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/574972/811067/porsci_lo__79508.1648973713__82089.1649091773.png?c=2 "CD74 Antibody [LN-2]")
![CD74 Antibody [LN-2], Mouse IgG1](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/823994/1115578/porsci_lo__79508.1648973713__16731.1649084691__20868.1649235586__20251.1649248189__37803.1657533733.png?c=2 "CD74 Antibody [LN-2], Mouse IgG1")
![CD74 Antibody [LN-2], Rabbit IgG](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/823995/1115579/porsci_lo__79508.1648973713__16731.1649084691__20868.1649235586__20251.1649248189__84899.1657533733.png?c=2 "CD74 Antibody [LN-2], Rabbit IgG")
![CD74 Antibody [BU45]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/574971/811066/porsci_lo__79508.1648973713__58985.1649091773.png?c=2 "CD74 Antibody [BU45]")