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![CK18 Antibody [KRT18/836]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575272/811767/porsci_lo__79508.1648973713__28388.1649091877.png?c=2 "CK18 Antibody [KRT18/836]")
![CK18 Antibody [KRT18/836]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575272/811767/porsci_lo__79508.1648973713__28388.1649091877.png?c=2 "CK18 Antibody [KRT18/836]")
Product Description
CK18 Antibody [KRT18/836] | 33-432 | ProSci
Host: Mouse
Reactivity: Human, Dog, Cat
Homology: N/A
Immunogen: Recombinant human KRT18 protein was used as the immunogen for the CK18 antibody.
Research Area: Signal Transduction
Tested Application: Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 2-4 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the CK18 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: Does not react with rat
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: KRT18/836
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
![CK18 Antibody [KRT18/836]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575272/811767/porsci_lo__79508.1648973713__28388.1649091877.png?c=2 "CK18 Antibody [KRT18/836]")
