Product Description
EGF Receptor Antibody [B1D8] | 33-120 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Microsomes from A431 cells were used as the immunogen for this antibody.
Research Area: Cancer, Signal Transduction
Tested Application: Flow, IP, Inhibition
Application: Functional Activity (Inhibition) : antibody blocks EGF receptor activation
inhibits A431 cell proliferation
arrests tumor growth in vivo
affinity purification
IP: 1-2µg/500ug protein lysate
Flow Cytometry: 0.5-1 ug/million cells
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Specificiy: Does not react with mouse and rat
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G purified monoclonal antibody
Clonality: Monoclonal
Clone: B1D8
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF) , Transforming Growth Factor-a (TGFa) , Heparin-binding EGF (HB-EGF) , amphiregulin, betacellulin and epiregulin.