Euro
USD
British Pound
NULL
![GAPDH Antibody [1D4]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/553229/761150/porsci_lo__79508.1648973713__96292.1649086179.png?c=2 "GAPDH Antibody [1D4]")
![GAPDH Antibody [1D4]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/553229/761150/porsci_lo__79508.1648973713__96292.1649086179.png?c=2 "GAPDH Antibody [1D4]")
Product Description
GAPDH Antibody [1D4] | 50-267 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig
Homology: N/A
Immunogen: Extensively purified pig GAPDH.
Research Area: Cancer
Tested Application: WB, IHC
Application: N/A
Specificiy: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 38
Validation: N/A
Isoform: N/A
Purification: Total IgM fraction--concentrated culture supernatant
Clonality: Monoclonal
Clone: 1D4
Isotype: IgM
Conjugate: Unconjugated
Physical State: Liquid
Buffer: N/A
Concentration: N/A
Storage Condition: GAPDH antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
![GAPDH Antibody [1D4]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/553229/761150/porsci_lo__79508.1648973713__96292.1649086179.png?c=2 "GAPDH Antibody [1D4]")
![Rhodopsin Antibody [1D4]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/553137/760966/porsci_lo__79508.1648973713__40470.1649086155.png?c=2 "Rhodopsin Antibody [1D4]")
![Glyceraldehyde 3 phosphate Dehydrogenase (GAPDH) Antibody [1D4] | MC-993](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/391261/569724/klogo__76714.1643661101__89857.1644059237.png?c=2 "Glyceraldehyde 3 phosphate Dehydrogenase (GAPDH) Antibody [1D4] | MC-993")
