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![ICAM3 Antibody [ICAM3/1019]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575248/811715/porsci_lo__79508.1648973713__28786.1649091871.png?c=2 "ICAM3 Antibody [ICAM3/1019]")
![ICAM3 Antibody [ICAM3/1019]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575248/811715/porsci_lo__79508.1648973713__28786.1649091871.png?c=2 "ICAM3 Antibody [ICAM3/1019]")
Product Description
ICAM3 Antibody [ICAM3/1019] | 33-402 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human protein was used as the immunogen for the ICAM3 antibody.
Research Area: Immunology, Signal Transduction
Tested Application: Flow, IF, WB, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the ICAM3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: ICAM3/1019
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
![ICAM3 Antibody [ICAM3/1019]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575248/811715/porsci_lo__79508.1648973713__28786.1649091871.png?c=2 "ICAM3 Antibody [ICAM3/1019]")
![ICAM3 Antibody [CDLA50-1]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575742/812920/porsci_lo__79508.1648973713__42019.1649092027.png?c=2 "ICAM3 Antibody [CDLA50-1]")
