Product Description
IL-11 Antibody [27B1047.1] | XP-5707-M | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human IL-11 as the immunizing antigen.
Research Area: Immunology, Chemokines & Cytokines, Neuroscience, Functional Assays
Tested Application: E, WB, Neut
Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL) , a concentration of 2.4 - 3.6 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11 (XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL.
Western blot:
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
Clonality: Monoclonal
Clone: 27B1047.1
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: IL-11 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Alternate Name: Interleukin 11Interleukin-11, Adipogenesis inhibitory factor, IL-11, AGIF
User Note: Centrifuge vial prior to opening.
BACKGROUND: N/A