Product Description
LAL Antibody [9G7F12] | 32-185 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant LAL expressed in E. Coli strain BL21 (DE3) .
Research Area: Homeostasis
Tested Application: E, WB
Application: Western Blot:Dilution 1:500 - 1:2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A affinity chromatography.
Clonality: Monoclonal
Clone: 9G7F12
Isotype: IgG2a
Conjugate: Unconjugated
Physical State: N/A
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: LAL monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Cholesteryl esterase, Acid cholesteryl ester hydrolase, LAL, CESD
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Lysosomal acid lipase (LAL) , with 378-amino acid protein ( 43-54 kDa) , functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides which are taken up by receptor-mediated endocytosis. An inherited deficiency or low activity of human lysosomal acid lipase results in the intralysosomal storage of the respective lipid substrates. So it is also responsible for the rare conditions of Wolman disease and cholesteryl ester storage disease (CESD) . As the enzyme is synthesized by all nucleated cells, lipid-laden cells are found in all organs, particularly in liver, spleen, the adrenal and the hemopoietic system, and in the intestine as well as in the lymph nodes, lungs, testes, and ovaries.