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![Melanoma Antibody [PNL2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575363/811970/porsci_lo__79508.1648973713__02975.1649091903.png?c=2 "Melanoma Antibody [PNL2]")
![Melanoma Antibody [PNL2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575363/811970/porsci_lo__79508.1648973713__02975.1649091903.png?c=2 "Melanoma Antibody [PNL2]")
Product Description
Melanoma Antibody [PNL2] | 33-528 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Dog
Homology: N/A
Immunogen: Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
Research Area: Cancer
Tested Application: WB, IF, IHC-P
Application: Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: PNL2
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%) , as opposed to 76% for anti-HMB45 and 82% for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
![Melanoma Antibody [PNL2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575363/811970/porsci_lo__79508.1648973713__02975.1649091903.png?c=2 "Melanoma Antibody [PNL2]")
