Product Description
MyD88 Antibody [2E9C2] | PM-4673 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2: Cat. No. 95-104 - MyD88 Recombinant Protein
Positive Control 3: Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: MyD88 Monoclonal Antibody is Protein A purified.
Clonality: Monoclonal
Clone: 2E9C2
Isotype: IgG2b
Conjugate: Unconjugated
Physical State: Liquid
Buffer: MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: MyD88 monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: MyD88 Antibody [2E9C2] : MYD88D
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.