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![TMEM16A Antibody [DG1/447]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575094/811370/porsci_lo__79508.1648973713__39866.1649091812.png?c=2 "TMEM16A Antibody [DG1/447]")
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Product Description
TMEM16A Antibody [DG1/447] | 33-234 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant human TMEM16A/DOG1 protein was used as the immunogen for this TMEM16A antibody.
Research Area: Signal Transduction
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TMEM16A antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: DG1/447
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST’s) , c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for TMEM16A has been reported in 97.8 percent of scorable GIST’s, including all c-kit negative GIST’s. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST’s is nearly identical: 94.4% vs. 94.7%.
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