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![TOP1MT Antibody [TOP1MT/488]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575188/811586/porsci_lo__79508.1648973713__26506.1649091852.png?c=2 "TOP1MT Antibody [TOP1MT/488]")
![TOP1MT Antibody [TOP1MT/488]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575188/811586/porsci_lo__79508.1648973713__26506.1649091852.png?c=2 "TOP1MT Antibody [TOP1MT/488]")
Product Description
TOP1MT Antibody [TOP1MT/488] | 33-332 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.
Research Area: Other
Tested Application: WB, Flow, IF, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the TOP1MT antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: TOP1MT/488
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
![TOP1MT Antibody [TOP1MT/488]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575188/811586/porsci_lo__79508.1648973713__26506.1649091852.png?c=2 "TOP1MT Antibody [TOP1MT/488]")
![TOP1MT Antibody [TPIMT-1]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575688/812783/porsci_lo__79508.1648973713__85137.1649092009.png?c=2 "TOP1MT Antibody [TPIMT-1]")
