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Product Description
Vinculin Antibody [7E10] | PM-7811 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat, Rabbit, Chicken
Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
The immunogen is located within amino acids 50 - 100 of Vinculin.
Research Area: Homeostasis
Tested Application: E, WB
Application: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Specificiy: Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Positive Control 1: Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 117 kDa
Observed: 120 kDa
Validation: N/A
Isoform: N/A
Purification: Vinculin antibody is purified via Protein A prification.
Clonality: Monoclonal
Clone: [7E10]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: CMD1W, CMH15, Metavinculin, MVCL
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1) . Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2) . Expression of vinculin were shown to be affected by the level of actin expression (2, 3) . Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4) . Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5) .
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