Product Description
Bcl-2 Antibody [10C4] | 99-624 | ProSci
Host: Mouse
Reactivity: Mouse, Rat
Homology: N/A
Immunogen: Murine Bcl-2 peptide (a.a. 61-76)
Research Area: Cancer, Obesity, Signal Transduction
Tested Application: Flow
Application: Bcl-2 Antibody [10C4] for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and immunoprecipitation assays.
Specificiy: Bcl-2
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: [10C4]
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration: 0.1 mg/mL
Storage Condition: Store vial at 2-8˚C
Alternate Name: B cell leukemia 2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membrane-bound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2’s ability to promote cell survival.