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![E. coli O157 Antibody [9.88]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575493/812287/porsci_lo__79508.1648973713__68698.1649091942.png?c=2 "E. coli O157 Antibody [9.88]")
![E. coli O157 Antibody [9.88]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575493/812287/porsci_lo__79508.1648973713__68698.1649091942.png?c=2 "E. coli O157 Antibody [9.88]")
Product Description
E. coli O157 Antibody [9.88] | 33-674 | ProSci
Host: Mouse
Reactivity: E. coli
Homology: N/A
Immunogen: A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.
Research Area: Infectious Disease
Tested Application: E, IF
Application: ELISA: 1-5 ug/ml; order BSA free formulation for coating
IF: 1-2 ug/ml
Optimal dilution of the E. coli O157 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: PEG precipitation followed by dialysis in 1X PBS
Clonality: Monoclonal
Clone: 9.88
Isotype: IgM, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC) , enteroinvasive E. coli (EIEC) , enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
![E. coli O157 Antibody [9.88]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575493/812287/porsci_lo__79508.1648973713__68698.1649091942.png?c=2 "E. coli O157 Antibody [9.88]")
