Product Description
Filaggrin Antibody [SPM181] | 33-724 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: A recombinant human FLG protein was used as the immunogen for this Filaggrin antibody.
Research Area: Stem Cell
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SPM181
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: FLI1 Antibody : ATOD2, Filaggrin
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Filaggrin (filament aggregating protein) is a filament-associated protein that binds to keratin fibers in epithelial cells. Ten to twelve filaggrin units are post-translationally hydrolized from a large profilaggrin precursor protein during terminal differentiation of epidermal cells. In humans, profilaggrin is encoded by the FLG gene, which is part of the S100 fused-type protein (SFTP) family within the epidermal differentiation complex on chromosome 1q21.
Filaggrin is essential for the regulation of epidermal homeostasis. Within the stratum corneum, filaggrin monomers can become incorporated into the lipid envelope, which is responsible for the skin barrier function. Alternatively, these proteins can interact with keratin intermediate filaments. Filaggrin undergoes further processing in the upper stratum corneum to release free amino acids that assist in water retention. [Wiki]