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Product Description
ICAM1 Antibody, Monoclonal | 51-076 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: ICAM1 monoclonal antibody was raised against Human monocytes (Human) .
Research Area: Immunology
Tested Application: IHC, IP, Flow
Application: ICAM1 antibody can be used in Western Blot starting at 0.5 - 1 μg/mL, and immunohistochemistry starting at 5 μg/mL. Flow Cytometry 1:50-1:100 Immunohistochemistry (frozen) - 1:50-1:100, Immunohistochemistry (FFPE) , Immunoprecipitation. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry Recommended positive control tissue: Human Tonsil. Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function. Removal of sodium azide is recommended prior to use in functional assays.
Specificiy: Species Crossreactivity: Clone 3H1547 is reported to crossreact with porcine endothelium.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G Column
Clonality: Monoclonal
Clone: N/A
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS, pH 7.4, 0.09% sodium azide
Concentration: 1 mg/ml
Storage Condition: Store ICAM1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Alternate Name: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ICAM1 Antibody, Monoclonal
