Product Description
Involucrin Antibody [INVO49] | 33-944 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Purified protein was used as the immunogen for the Involucrin antibody.
Research Area: Cancer, Signal Transduction
Tested Application: IF, IHC-P
Application: Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 0.1-0.2 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Involucrin antibody may be required for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: INVO49
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: IVL,
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: Involucrin is a highly reactive, soluble, transglutaminase substrate protein present in keratinocytes of epidermis and other stratified squamous epithelia. It first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase thus helping in the formation of an insoluble envelope beneath the plasma membrane functioning as a glutamyl donor during assembly of the cornified envelope. [Wiki]