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Product Description
SOX2 Antibody [SOX2/1792] | 33-742 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Amino acids 176-305 of the human protein were used as the immunogen for the SOX2 antibody.
Research Area: Homeostasis, Stem Cell
Tested Application: E, WB, IHC-P
Application: ELISA: 2-4 ug/ml; order BSA-free formulation for coating
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the SOX2 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SOX2/1792
Isotype: IgG2b, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.
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