Product Description
TCRab/Vb1 Antibody [TCR-2] (PE) | 99-226 | ProSci
Host: Mouse
Reactivity: Chicken, Turkey, Peacock, Guinea Fowl
Homology: N/A
Immunogen: White Leghorn chick thymocytes and blood mononuclear cells
Research Area: Immunology
Tested Application: E, ICC, Flow, IP
Application: TCRab/Vb1 Antibody [TCR-2] for use in flow cytometry assays.
Specificiy: TCRalphabeta/Vbeta1
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: [TCR-2]
Isotype: IgG1
Conjugate: PE
Physical State: Liquid
Buffer: Supplied in PBS/NaN3 and a stabilizing agent
Concentration: 0.1 mg/mL
Storage Condition: Store vial at 2-8˚C
Alternate Name: T3/TCR complex, TCR alpha/beta
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The monoclonal antibody TCR-2 precipitates a CD3-associated heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 29 kDa. In the chicken, two distinct subpopulations of alphabeta T cells appear in the thymus subsequent to the appearance of gammadelta T cells. These subpopulations, originally denoted as TCR2 and TCR3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. The TCR-2 monoclonal antibody reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells, and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74 ± 2%) or CD8 (26 ± 4%) . TCR2+ cells in the spleen also express either CD4 (37 ± 1%) or CD8 (64 ± 4%) . Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2. This observation led to the demonstration that 71 ± 6% of the TCRgammadelta+ cells in the spleen express CD8.